【英文标准名称】:StandardGuideforConductingRenewalMicroplate-BasedLife-CycleToxicityTestswithaMarineMeiobenthicCopepod
【原文标准名称】:用海洋Meiobenthic桡足类甲壳动物进行复原的基于微板的寿命周期毒性试验的标准指南
【标准号】:ASTME2317-2004
【标准状态】:现行
【国别】:
【发布日期】:2004
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E47.01
【标准类型】:(Guide)
【标准水平】:()
【中文主题词】:
【英文主题词】:microplate-based;life-cycle;toxicitytests;marine;meiobenthic;copepod
【摘要】:1.1Thisguidedescribesproceduresforobtaininglaboratorydataconcerningtheadverseeffectsofatestmaterialaddedtoseawater,butnottofood,onthemarinecopepodAmphiascustenuiremis,duringcontinuousexposuresofindividuals,fromimmediatelyafterbirth,untilafterthebeginningofreproductionusinga200956;Lrenewalmicroplate-culturingtechnique.Thefollowingdataarecheckedandrecordedduringthetestperiod:stage-specificsurvival,numberofdaysittakesfordevelopmentfromafirststagenaupliustoareproductivelymaturecopepod,genderratios,numberofdaysforafemaletoextrudefirstandsubsequentbroods,numberofdaysbetweenfirst(andsubsequent)broodextrusion(s)andhatchingoffirst-generationnauplii,numberofhatchedandsurvivingnauplii,numberofunhatchedornecroticeggsandabortedunhatchingeggsacs,andthetotalnumberoffemalesabletoproduceviableoffspringovertheentirematingperiod.Thismicroplate-basedfulllife-cycletoxicitytesthasadurationofapproximately17daysfortoxicantsthatdonotdelaydevelopment.Theseproceduresprobablywillbeusefulforconductinglife-cycletoxicitytestswithotherspeciesofcopepods,althoughmodificationsmightbenecessary.1.2Theseproceduresareapplicabletomostchemicals,eitherindividually,orinformulations,commercialproducts,orknownmixtures,thatcanbemeasuredaccuratelyatthenecessaryconcentrationinwater.Withappropriatemodificationstheseprocedurescanbeusedtoconducttestsontemperature,dissolvedoxygen,andpHandonsuchmaterialsasaqueouseffluents(seealsoGuideE1192),sedimentporewaters,andsurfacewaters.Renewalmicroplatetestsmightnotbeapplicabletomaterialsthathaveahighoxygendemand,arehighlyvolatile,arerapidlytransformed(biologicallyorchemically)inaqueoussolutions,orareremovedfromtestsolutionsinsubstantialquantitiesbythetestchambersororganismsduringthetest.Iftheconcentrationofdissolvedoxygenfallsbelow50%ofsaturation,ortheconcentrationoftestmaterialinthetestsolutiondecreasesbymorethan20%betweenrenewals,itmightbedesirabletorenewthesolutionsmoreoften.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatoryrequirementspriortouse.
【中国标准分类号】:Z16
【国际标准分类号】:07_100_99
【页数】:16P.;A4
【正文语种】: